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EC number: 203-095-9
CAS number: 103-28-6
The Adsorption Coefficient of test substance
was determined as per the HPLC method (OECD Guideline-121). The Log Koc
value was determined to be 2.474± 0.001 at 25°C. Thus based on the
result it is concluded that the test substance has a low sorption to
soil and sediment and therefore has moderate migration potential to
The adsorption coefficient Koc in soil and
in sewage sludge of test chemical was determined by the Reverse Phase
High Performance Liquid Chromatographic method according to OECD
Guideline No. 121 for testing of Chemicals. The solutions of the test
substance and reference substances were prepared in appropriate solvents.
A test item solution was prepared by
accurately pipetting 4 microliter of test item and diluted with
Acetonitrile up to 10 ml. Thus, the test solution concentration was
426.4 mg/l. The pH of test substance was 5.8
Each of the reference substance and test
substance were analysed by HPLC at 210 nm. After equilibration of the
HPLC system, Urea was injected first, the reference substances were
injected in duplicate, followed by the test chemical solution in
duplicate. Reference substances were injected again after test sample,
no change in retention time of reference substances was observed.
Retention time tR were measured, averaged and the decimal logarithms of
the capacity factors k were calculated. The graph was plotted between
log Koc versus log k(Annex - 2).The linear regression parameter of the
relationship log Koc vs log k were also calculated from the data
obtained with calibration samples and therewith, log Koc of the test
substance was determined from its measured capacity factor. The
Benzoic acid methylester, Xylene,
Ethylbenzene, Toluene, Naphthalene, Benzoic acid phenylester were chosen
having Koc value range from 1.8 to 2.87.The Log Koc value of test
chemical was determined to be 2.474± 0.001 dimensionless at 25°C.This
log Koc value indicates that the substance has a lowsorption to soil and
sediment and therefore has moderate migration potential to ground water.
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