N,N-bis(2-methylpropyl)formamide

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1988-04-27 to 1988-05-11

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Basic data given. Only orientating information about the inhalation hazard.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

BASF-Test (IRT = inhalation risk test):
The test was performed in principle as described in OECD test guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of the test substance at the temperature chosen for vapor generation (50°C). Rats were exposed to the test substance vapors for 8 hours. Documentation of clinical signs was performed over the 7-day study period.

GLP compliance:

no

Test type:

other: Inhalation hazard test

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N,N-bis(2-methylpropyl)formamide

EC Number:

219-983-4

EC Name:

N,N-bis(2-methylpropyl)formamide

Cas Number:

2591-76-6

Molecular formula:

C9H19NO

IUPAC Name:

N,N-bis(2-methylpropyl)formamide

Test material form:

other: liquid

Details on test material:

- Name of the test substance used in the study report: Diisobutylformamid
- Purity: 99.7 %

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, 7950 Biberach, Germany
- Age at study initiation: about 8 - 9 weeks
- Weight at study initiation (mean): males: 262 g, females: 188 g
- Housing: They were housed in groups of three in type D III wire mesh cages supplied by Becker & Co., without bedding.
- Diet: KLIBA 24-343-4 rat/mouse laboratory diet (10 mm pellets, Klingentalmühle AG, CH-4303 Kaiseraugst, Switzerland), ad libitum in the exposure-free period.
- Water: ad libitum in the exposure-free period
- Acclimation period: At least 5 days
- Animal identification: colour lines on the base of the tail

ENVIRONMENTAL CONDITIONS
- Temperature: 20 - 24 °C
- Humidity: 30- 70 %
- Photoperiod: 12 hours dark/ 12 hours light

Administration / exposure

Route of administration:

other: mixture of the vapour of the test substance and air

Vehicle:

other: unchanged

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus, filled volume: Glass bottle (fritted glass flask, pore-size 90 - 150 um, diameter 30 mm) filled to a height of 5 cm with the test substance
- Source and rate of air: compressed air, 200 L/h
- Treatment of exhaust air: Disposal
- Temperature in air chamber: Between 19 and 25 °C

Duration of exposure:

7 h

Concentrations:

The mean concentration of the test substance (calculated for a study duration of 7 hours) was 0.43 mg/L.

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Clinical examinations took place each workday. Lethality was checked each day.
- Necropsy of survivors performed: yes

Results and discussion

Mortality:

No mortality was observed.

Clinical signs:

other: During exposure: intermittently breathing, increased masticatory movement, snout wiping After exposure: nothing abnormal detected

Body weight:

Not determined.

Gross pathology:

No treatment related effects were seen during necropsy.