2-{[4-({4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulfo-1-naphthyl}diazenyl)-(6 or 7)-sulfo-1-naphthyl]diazenyl}benzene-1,4-disulfonic acid, lithium sodium salts

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

The main structure of the read-across substance is the same of the CJ313, only the salt form is different. However, this difference does not influence the toxicity of the CJ313. CJ313 contains Li/Na salt form and the read-across substances contains only Na salt. This will not lead to difference on the toxicity results.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

None

Vehicle:

not specified

Details on test solutions:

None

Test organisms (species):

activated sludge

Details on inoculum:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

None

pH:

None

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

100.0, 32.0 and 10.0 mg/l

Details on test conditions:

None

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

None

Results with reference substance (positive control):

The IC50 of reference substance (3,5-Dichlorophenol) = 13 mg/l.

Reported statistics and error estimates:

None

Validity criteria fulfilled:

not specified

Conclusions:

The IC50 for FAT 40032/D was found to be greater than 400 mg/L.

Executive summary:

A key toxicity study of FAT 40032/D to activated sludge was determined in a 3-hours respiration inhibition test following methodology similar to OECD Guideline 209. At 10, 32 and 100 mg/L concentrations, respiratory inhibitions observed were 0, 6.3 and 15.8 % respectively. Based on the findings of the study, it was concluded that the IC50 (3 h) of the test substance FAT 40032/D is greater than 400 mg/L.

Description of key information

The IC50 for FAT 40032/D was found to be greater than 400 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

400 mg/L

Additional information

The IC50 for Reactive Brown 11 was reported to be >300 mg/L in the four available study reports.

In the key toxicity study of FAT 40032/D to activated sludge, conducted according to methodology similar to OECD Guideline 209. In this 3-hours respiration inhibition test, the test substance concentrations tested were 100.0, 32.0 and 10 mg/l. Based on the findings of the study, the IC50 (3 h) of the test substance FAT 40032/D was found to be >400 mg/L.