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EC number: 201-121-3
CAS number: 78-50-2
Micronucleated polychromatic erythrocytes
Time following second dose
Number of mice
Number per 1000 PCE’s (mean±SD)
Number per PCE’s scored
Corn oil 10 mL/kg/d
Test substance (476 mg/kg bw/d)
Test substance (159 mg/kg bw/d)
Test substance (48 mg/kg bw/d)
Triethylenemelamine (TEM) (0.15 mg/kg bw/d)
An OECD Guideline
(474) comparable study was conducted to assess the genotoxicity of the
read across substance ‘A mixture of: hexyldioctylphosphineoxide;
dihexyloctylphosphineoxide; trioctylphosphineoxide’ using a micronucleus
cytogenetic assay in mice. The test substance-vehicle mixture and the
vehicle alone were administered by intraperitoneal (IP) injection in two
equal volumes of 10 mL/kg bw/d which were given 24 h apart. Positive
control was injected through IP route at a dose level of 0.15 mg/kg bw.
At the sacrifice (6 h following second dose), femurs were exposed, cut
just above the knee and the bone marrow was aspirated into a syringe
containing FBS. Using oil immersion, 1,000 polychromatic erythrocytes
were scored for the presence of micronuclei. The proportion of
polychromatic erythrocytes to total erythrocytes and the number of
micronucleated normocytes in the field of 1,000 polychromatic
erythrocytes were also enumerated. There was no significant difference
in the ratio of polychromatic erythrocytes to total erythrocytes between
treatment groups per sacrifice time. The number of micronucleated
polychromatic erythrocytes per 1,000 PCE was not significantly increased
in the test substance-treated groups, regardless of sex or sacrifice
time point. TEM induced a significant increase in micronucleated
polychromatic erythrocytes in male and female mice relative to the
vehicle control. Under the study conditions, the read across substance
did not increase the frequency of micronuclei in mice (Putman DL, 1986).
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