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EC number: 201-121-3
CAS number: 78-50-2
A study was
conducted to determine the toxicity of the test substance to
microorganisms using activated sludge respiration inhibition test
according to OECD Guideline 209 and EU Method C.11, in compliance with
GLP. The study was conducted under static conditions using freshwater
media. Test organisms were exposed to the test substance at loading
rates of 0, 10, 100 and 1,000 mg /L. The total exposure period was 3 h
and the oxygen consumption was recorded for a period of approximately 10
minutes post exposure. 3,5-dichlorophenol was used as positive control.
No inhibition of the respiration rate of the sludge was recorded at any
of the loading rates. The EC50of 3,5-dichlorophenol was in
the accepted range of 2 to 25 mg/L for total respiration (7.8 mg/L).
Under the study conditions, the test substance was not toxic to waste
water bacteria (activated sludge) at a loading rate of 1,000 mg/L
(NOEL). The EL50was therefore above 1,000 mg/L (Soeter M,
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