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EC number: 201-121-3
CAS number: 78-50-2
inhibition test was performed to evaluate the toxicity of the test
substance to alga, Selenastrum capricornutum, according to OECD
Guideline 201, in compliance with GLP. In this test, 3 replicates, each
containing approximately 1.0 E4 cells/mL were exposed to
water accommodated fractions (WAFs) of the test substance at nominal
concentrations of 0, 1, 50, 100, 200 and 300 mg/L (equivalent to
measured concentrations of 0, 0, 0, 1.29 and 3.98 mg/L, respectively)
under static conditions for a period of 96 h. Growth (biomass) and
growth rate were recorded at 72 and 96 h. Calculations were performed
based on nominal loading rates as well as the calculated measured
concentrations of test substance. The analytical concentration of the
test substance was measured by the Dissolved Organic Carbon (DOC)
method. Under the study conditions, the NOEC and LOEC values for both
growth rate and growth (biomass) were 1 and 50 mg/L, respectively, based
on nominal loading rates. The 96 h ErC50 was 238
mg/L (nominal), equivalent to a measured concentration of 1.53 mg/L. The
96 h EbC50was 162 mg/L, based on nominal loading
rate, equivalent to a measured concentration of 1.87 mg/L (Bragin GE,
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