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Short-term toxicity to aquatic invertebrates

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An acute immobilization test was performed to evaluate the toxicity of the test substance to Daphnia magna according to OECD Guideline 202, in compliance with GLP. In this test, 4 replicates of 5 Daphnia magna neonates were exposed to water accommodated fractions (WAFs) of the test substance at nominal concentrations of à, 62.5, 125, 250, 500 and 1,000 mg/L (i.e., equivalent to measured concentrations of 0.96, 2.77, 0.84, 3.46 and 9.32 mg/L, respectively) under static conditions for 48 h. Immobility was recorded at 24 and 48 h. The analytical concentration of the test substance was measured by the Dissolved Organic Carbon (DOC) method at 0 and 48 h. The EC50values were 503 mg/L (with 95% CI of 411 to 637 mg/L) based on nominal loading rates and 4.11 mg/L (with 95% CI of 3.33 to 5.55 mg/L) based on measured values, respectively. Immobilization was observed in all treatments (including the control), hence there was no maximum concentration causing no immobilization. Under the study conditions, the 48 h EC50 of the test substance was 503 mg/L (i.e. equivalent to 4.11 mg/L measured concentrations) (Bragin GE, 1993b).