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Biodegradation in water: screening tests

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Description of key information

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A study was performed to assess the ready biodegradability of the test substance according to OECD 301D, in compliance with GLP. The substance was weighed onto a glass fibre filter. The filter was then transferred to individual BOD bottles to which test medium was added to achieve a concentration of 2 mg/L. Since the test substance was water insoluble, actual concentrations were dependent on addition of neat test material to individual BOD bottles, and ranged from 2.00 to 4.67 mg/L. The test substance was biodegraded to 62.6% after 28 d. Since aniline, the positive control material, exceeded the pass level by degrading to 92.2%, this test can be considered valid. Under the study conditions, the test substance was considered to be readily biodegradable (Lee C, 1992).