3-trimethoxysilylpropyl methacrylate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

Detail missing on animals and environmental conditions.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-trimethoxysilylpropyl methacrylate

EC Number:

219-785-8

EC Name:

3-trimethoxysilylpropyl methacrylate

Cas Number:

2530-85-0

Molecular formula:

C10H20O5Si

IUPAC Name:

3-trimethoxysilylpropyl methacrylate

Test material form:

liquid

Test animals

Species:

rat

Strain:

Fischer 344

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS

- Source: Charles River Breeding Laboratories (Kingston, NY)

- Age at study initiation: ca. 6 weeks

- Housing: Stainless steel cages, with wire floors

- Diet: Agway certified rodent chow

- Water: provided by an automatic water system

- Acclimation period: 2 weeks

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

other: distilled water adjusted to pH4 with acetic acid

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure chamber volume: 120 liters

- System of generating particulates/aerosols: Four solo-sphere nebulizers containing a 15% (w/w) solution of 3-(trimethoxysilyl)propyl methacrylate (in distilled water, adjusted to pH 4 with acetic acid) were connected to 2 large triple-necked flasks (to trap large liquid droplets) which were, in turn, connected to the exposure chamber. Dried air was passed into each nebulizer, operating at a pressure of 20 p.s.i, and the resulting aerosol conducted into the animal chamber.

- Method of particle size determination: One sample of chamber atmosphere was analysed for particle size. A 3 minute flow of air at 10 l/min was conducted through a Sierra Cascade Inspector. Particles were collected on 3 stages of glass fibre filters. Several chamber air samples were subjected to gas chromatographic analysis to determine methanol concentration.

TEST ATMOSPHERE

- Brief description of analytical method used: Every 30 minutes during exposure, a sample of chamber atmosphere was drawn through a tared fiberglass filter at the rate of 2.2 l/min. After 2 min the filter was removed, dried in a drying overn to a constant weight and the final weight recorded. The change in weight represented the amount of hydrolysed silane collected. According to the sponsor, this represents ca. 72.17% of the test substance. This factor, along with the volume of air sampled, was used to calculate the concentration of test substance in the chamber air.

- Samples taken from breathing zone: yes

VEHICLE

- Composition of vehicle (if applicable): distilled water

- Concentration of test material in vehicle (if applicable): Distilled water was adjusted to pH 4 with acetic acid and used to make a 15% solution of test substance.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

GC

Duration of exposure:

4 h

Concentrations:

2.28 mg/L

No. of animals per sex per dose:

5 males, 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

- Frequency of observations and weighing: The animals were weighed just before exposure and at 7 and 14 days after exposure. All rats were observed frequently on the day of the test and daily during the subsequent observation period.

- Necropsy of survivors performed: yes

Statistics:

No statistical analysis was included in the report.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 2.28 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

There were no deaths.

Clinical signs:

other: During exposure, a dense fog was present in the animal chamber, preventing observation of the test rats. After exposure, slow righting reflex and laboured breathing were apparent. At one day, the animals were fully recovered.

Body weight:

All animals gained weight.

Gross pathology:

No remarkable gross pathologic findings were seen.

Other findings:

None reported.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In an acute inhalation toxicity study, conducted in a similar manner to OECD 403 but pre-GLP, an LC50 value of >2.28 mg/l (aerosol exposure) was determined.