2,3-epoxypropyl o-tolyl ether

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 121 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Test material information

Constituent 1

Reference substance name:

2,3-epoxypropyl o-tolyl ether

EC Number:

218-645-3

EC Name:

2,3-epoxypropyl o-tolyl ether

Cas Number:

2210-79-9

Molecular formula:

C10H12O2

IUPAC Name:

oxirane

Constituent 2

Reference substance name:

Oxirane, 2-[(2-methylphenoxy)methyl]-

IUPAC Name:

Oxirane, 2-[(2-methylphenoxy)methyl]-

Details on test material:

As per IUCLID Sections 1.1. 1.2. and 4.1.

Radiolabelling:

no

Study design

Test temperature:

Ambient

HPLC method

Details on study design: HPLC method:

Approximately 0.08 g of test substance was placed in a 20 mL LSC vial and brought to volume with mobile phase (55% methanol:45% water). A calibration curve using structurally related reference compounds of known Log Koc was established, using methanol or mobile phase. The test substance and reference compounds of known Log Koc. were injected into an HPLC system. A calibration curve using retention time (Log t) versus Logo Koc. was calculated by linear regression analysis from the injection data of the reference compounds. The calibration curve was calculated from literature values for the Log Koc of the reference compounds and the corresponding capacity factor, k, calculated as:

K = (tr-to) / to where tr is the retention time of the reference compound

From this curve, the unknown Log Koc of the test substance was estimated from its retention time by making injections of small samples of the test substance following identical column, elution, and detection parameters used to establish the calibration curve. The Log Koc of the test substance was determined by calculating the capacity factor (k) of the test substance.

The HPLC system used for the analysis consisted of: Varian Prostar HPLC with Varian Model 410 autosampler equipped with a partial loop injector. Data capture was by Galaxie System software. The colunm was Phenomenex Luna C18 with 5 jam particle size x 250 mm length x 4.6 mm inner diameter; Phenomenex Cyano 5 um particle size x 250 mm length x 4.6 mm inner diameter for Log Koc.

The HPLC operational parameters were as follows: Detector; PDA, Flow rate (mobile phase); 0.8 mL/min. Injection volume; 10 1ul, Detector wavelength; 210 nm, Colunm temperature; Room Temperature, Attenuation / Chart Speed; Autoscale, Threshold / Peak Width 1000 / 2.0 and Mobile phase solvent; (55:45 ratio of methanol/water).

Results and discussion

Adsorption coefficient

Type:

Koc

Value:

ca. 210

Remarks on result:

other: Ambient temperature.

Results: HPLC method

Details on results (HPLC method):

The Log Koc of the test substance was determined to be 2.32 and the adsorption coefficient (Koc) was approximately 210 when estimated by comparing the retention times of the multiple reference compounds of known Log Koc. eluted through a reverse phase HPLC column.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance has a measured Log Koc of 2.32 demonstrating that it has a low to moderate ability to adsorb to sediment and soil relative to other more complex molecules (A. Delle Site. J. Phys. Chem. Ref. Data. 30: 2001, 187-439).

Executive summary:

The test substance, 2,3 -epoxypropyl o-tolyl ether, was evaluated for its Adsorption Coefficent by an O.E.C.D. test guideline 121 HPLC method.The test substance was determined to have a Log Koc of 2.32 demonstrating that it has a low to moderate ability to adsorb to sediment and soil relative to other more complex molecules (A. Delle Site. J. Phys. Chem. Ref. Data. 30: 2001, 187-439).