5,5'-(1H-isoindole-1,3(2H)-diylidene)dibarbituric acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: JIS K 0102 - 1974 item 55

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Cremophor RH 40 was used as dispering agent

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Orange-red Killifish
- Source: bred at testing facility. The animals were of Japanese origin (batch 9)
- Age at study initiation (mean and range, SD): 7 month
- Length at study initiation (length definition, mean, range and SD): 3.1 cm (range: 2.7 - 3.3 cm)
- Weight at study initiation (mean and range, SD): 241 mg
- Feeding during test: none


ACCLIMATION
- Acclimation period: 1 week
- Type and amount of food: "Tetra Min" standard feed for aquarium fish, Tetra-Werke, Melle, Germany; ad libitum

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

0.25 mmol/l

Test temperature:

23 °C

pH:

6.7 - 7.6

Dissolved oxygen:

5.2 - 8.1 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations (mg/L):
1470, 2150, 3160, 4640, 6810, 10000, 14700, 21500, 31600, control, 4300 solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria ((30 cm x 22 cm x 24 cm)
- Fill volume: 5 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to proposal on standard TLM test with Orange-red Killifish, Josuito Haisui (available water and waste water) 14, 1297-1303 (1972)
- Conductivity: max. 10 micro MHO


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 24, 48 and 96 hours.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 11 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The test solutions were coloured and cloudy. The solvent control turned cloudy after 48 hours.
Symptoms of intoxication were not observed.

Reported statistics and error estimates:

Finney (1971) Probit Analysis. Cambridge University Press, 3rd Edition

Description of key information

With high probability acutely not harmful to fish.

Additional information

In a guideline study following JIS K 0102 - 1974 item 55 the acute toxicity of the substance to fish was determined.Test fish of the species Oryzias latipes were exposed to the substance for 96 hours in a static test system. At test termination a LC50 of ca. 11000 mg/l based on nominal test substance concentrations was determined (BASF AG, 1983).