Dimethyl sulphate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally acceptad scientific standards, well documented and acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

- Animals were treated for 1, 5, and 15 min or 20 hours using occlusive conditions; - Skin tissue from the ear was also tested; - The test substance was washed out with water (which sometimes contained a mild detergent) after the exposure period.

Principles of method if other than guideline:

BASF-Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulphate

EC Number:

201-058-1

EC Name:

Dimethyl sulphate

Cas Number:

77-78-1

Molecular formula:

C2H6O4S

IUPAC Name:

dimethyl sulfate

Details on test material:

- Name of test material (as cited in study report): dimethyl sulfate
- Physical state: liquid

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: young adult animals
- Weight at study initiation: 2.35 kg (female; 2.46 kg at the end of the study) and 2.5 kg (male; 2.36 kg at the end of the study)

Test system

Type of coverage:

occlusive

Preparation of test site:

other: clipped and intact skin

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of the same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5-1 ml

Duration of treatment / exposure:

1 min, 5 min, 15 min and 20 hours

Observation period:

8 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: undiluted test substance was applied to a 2.5 x 2.5 cm patch
- Type of wrap if used: occlusive

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: up to 20 hours after treatment

SCORING SYSTEM:
Dermal reactions were classified using a BASF-scoring system, which is convertible into the current OECD Draize scheme.

Results and discussion

In vivo

Results

Irritation parameter:

other: the test substance is corrosive

Basis:

mean

Reversibility:

not reversible

Remarks on result:

other: necrosis was still observable at the end of the exposure period (8-day)

Irritant / corrosive response data:

Short time exposure caused no effects (1 min exposure) or light erythema (score: 2; 5 and 15 min exposure): Cutaneous reactions were reversible after 1 and 5 min exposure, but not after 15 min exposure at the end of the observation period.
Long time exposure (20 hours): Severe cutaneous reaction (erythema score 4 and edema score 3) and severe necrosis of skin tissue was reported for both animals 24 hours after application of the test substance, which persisted until the end of the observation period (8 days after application of the test substance).

Any other information on results incl. tables

Conclusion:

The test substance is considered to be corrosive based on the irreversible necrosis

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information