Registration Dossier
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EC number: 203-444-5 | CAS number: 106-93-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- not reported.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- publication
- Title:
- Further mutagenicity studies on pesticides in bacterial reversion assay systems.
- Author:
- Moriya, M., Ohta, T., Watanabe, K., Miyazawa, T., Kato, K. & Shirasu, Y.
- Year:
- 1 983
- Bibliographic source:
- Mutation Reseach., 1983, Vol. 116, p.185-216, ©Elsevier Biomedical Press.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Most of the pesticide samples were obtained from the Agricultural Chemicals Inspection Station of the Ministry of Agriculture, Forestry and Fisheries (Kodaira). They were standard materials obtainable in Japan. Pesticides soluble in water were dissolved in sterile distilled water, and all the other pesticides were dissolved in dimethyl sulfoxide (DMSO).
Method
- Target gene:
- Salmonella typhimurium: Histidine locus.
Escherichia coli: Tryptophan locus
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: TA1537 & TA98 frameshift mutation,
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- other: frameshift
- Species / strain / cell type:
- E. coli WP2
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- Pesticides were tested up to a dose of 5000 µg/plate, unless the compounds showed toxicity to bacteria at the dose. When a pesticide showed mutagenicity only at higher doses such as 1000-5000 µg/plate, the compound was tested at doses of more than 5000 µg/plate to obtain a dose-response relationship.
- Vehicle / solvent:
- Pesticides soluble in water were dissolved in sterile distilled water, and all the other pesticides were dissolved in dimethyl sulfoxide (DMSO).
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- Most procedures were performed according to the methods described by Ames et al. (1975). When the E. coil strain was used, histidine and biotin in top agar were replaced by tryptophan at the same concentration. To 2 ml of the top agar were added 0.1 ml of one of bacterial suspensions in phosphate buffer (1/15 M), 0.1 ml of a solution of a pesticide and, when required, 0.5 ml of the S9 mix (0.3 ml of S9 fraction/ml S9 mix). The contents were poured onto the surface of a minimal agar plate with modified Vogel-Bonnet E medium (Moriya et al., 1978). Plates were incubated at 37°C for 2 days, after which the number of revertant colonies on each plate was counted.
For the gaseous pesticide, methyl bromide, a plate overlaid with the top agar containing one of the strains with or without the S9 mix was placed upside down without a lid in a glass container. Gaseous methyl bromide was injected into the container from a syringe. The container was incubated at 37°C for 2 days with stirring of the inside air by a small electric fan.
Pesticides were tested up to a dose of 5000 /µg/plate, unless the compounds showed toxicity to bacteria at the dose. When a pesticide showed mutagenicity only at higher doses such as 1000-5000/µg/plate, the compound was tested at doses of more than 5000 µg/plate to obtain a dose-response relationship.
Ames, B.N., McCann, J. & Yamasaki, E. (1975) Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test, Mutation Res., Vol. 31, pp. 347-364.
Moriya, M., Kato, K. & Shirasu, Y. (1978) Effects of cysteine and a liver metabolic activation system on the activities of mutagenic pesticides, Mutation Res., Vol. 57, pp. 259-263. - Evaluation criteria:
- not reported.
- Statistics:
- not reported.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA1535, TA100, TA98
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium, other: TA1537, TA1538
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- E. coli WP2
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- The mutagenic action of EDB was not greatly affected by the addition of S9 mix.
- Remarks on result:
- other: strain/cell type:
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
The test material was found to be mutagenic to S. typhimurium and E. coli both with and without metabolic activation under the conditions of the test.
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