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EC number: 219-755-4
CAS number: 2524-04-1
study was conducted in order to identify possible mutagic activities of
EP-2 and was performed according to OECD guideline 471 and EU method
a preliminary toxicity test, Salmonella typhimurium strains TA98, TA100,
TA1535 and TA1537 and Escherichia coli strain WP2uvrA were treated at
EP-2 concentrations in the range of 0.15 - 5000 µg/plate to determine
the dose range to be tested.
same bacterial strains were treated with EP-2 in the presence and in the
absence of metabolic activation (S9 -mix) both in the plate
incorporation and in the pre-incubation assay with test concentrationsin
the range of 5 - 5000 µg/plate and 0.5 - 5000 µg/plate, respectively.
The tester strains without treatment or treated at the vehicle (acetone)
alone served as negative controls and appropriate positive controls
(with and withot S9 -mix) were examined in the same assays.
revealed counts of revertants in the normal range for vehicle control
plates. All of the positive control chemicals used in the assays induced
marked increases in the frequency of revertant colonies, both with or
without metabolic activation. Thus, the sensitivity of the assay and the
efficacy of the S9-mix were validated.
the first experiment (plate incorporation assay), EP-2 caused a visible
reduction in the growth of the bacterial background lawn in all of the
tester strains, both in the presence and absence of S9 -mix, initially
at 500 µg/plate. In the second experiment (pre-incubation assay), EP-2
caused a visible reduction in the growth of the bacterial background
lawn to all of the tester strains, with and without metabolic
activation, initially at 150 µg/plate. Thus, EP-2 is cytotoxic at
concentrations of 500 µg/plate and 150 µg/plate onwards in the plate
incorporation assay and the pre-incubation assay, respectively.
test item precipitate was observed on the plates at any concentration
tested either in the presence or in the absence of metabolic activation.
significant increases in the frequency of revertant colonies were
recorded for any of the bacterial strains, with any dose of EP-2 either
with or without metabolic activation or exposure method used. It is
therefore concluded that EP-2 (O,O-diethyl
In addition, in the IUCLID4 dataset on
O,O-diethyl phosphorochloridothioate, the results of an AMES test in
Salmonella typhimurium are presented and it is stated that the results
indicated that the test was negative both in the presence and in the
absence of metabolic activation. Thus, O,O-diethyl
phosphorochloridothioate is not mutagenic in this in vitro assay.
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