Reaction products of copper phthalocyanine, sulfuric acid, and sodium carbonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 08, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

no

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20.9 °C

pH:

7.6

Dissolved oxygen:

0.66 - 0.80 mg O2 pro min/l

Nominal and measured concentrations:

10, 32, 100, 320 mg/l

Reference substance (positive control):

yes

Remarks:

3.5 Dichlorphenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

IC50 (reference substance) = 9.2 mg/l

Control values:

control	pH(3h)	O2 - Consumption	Time	mgO2 pro min/l
K1	7.8	3.2	3.1	0.83	K1/K2 = 0.67%	ΦK = 0.79
K2	7.4	3	3.2	0.75	K2/K1 = 9.64%

Test substance values:

test substance	pH(3h)	O2 - Consumption	Time	mgO2 pro min/l	comparison with ΦK (%)	Consumption (%)
320	7.6	3.3	4.0	0.66	83.54	16.46
100	7.6	4.0	4.4	0.73	92.41	7.59
32	7.6	3.6	3.6	0.80	101.27	0.00
10	7.6	3.8	3.9	0.78	98.73	1.27

Reference substance values:

3.5 - dichlorophenol (mg/l)	pH(3h)	O2 - Consumption	Time	mgO2 pro min/l	comparison with ΦK (%)	Consumption (%)
32	7.6	0.8	4.9	0.13	16.46	83.54
10	7.5	1.9	4.2	0.36	45.57	54.43
3.2	7.5	3.7	4.5	0.66	83.54	16.46

Validity criteria fulfilled:

not specified

Conclusions:

IC50 (3h) > 320.0 mg/l

Executive summary:

The substance to be registered has been tested according to OECD 209, during the test the IC50 have been evaluated both for the test substance and for the 3,5 -dichlorophenol, used as reference substance.

For the test item IC50 > 320.0 mg/l, for the reference substance IC50 = 9.4.

Description of key information

Freshwater: IC50 (3h) > 320.0 mg/l

Key value for chemical safety assessment

EC50 for microorganisms:

320 mg/L

Additional information

Three studies are available for the toxicity to microorganisms but only the Huntsman one (1994) contains sufficient details to be considered reliable to be choose as key study. The other two are short abstracts which results are in agreement with the key study result. The key value for the risk assessment has been set as IC50 > 320 mg/l.