Registration Dossier
Registration Dossier
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EC number: 700-953-9 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Grow rate:
NOEC (0-72h): 1.0 mg/L
EC50(0-72h): 56.4 mg/L (45.0-63.7)
EC20(0-72h): 6.7 mg/L (3.4-14.0)
EC10(0-72h): 3.3 mg/L (1.4-5.4)
Yeald::
NOEC (0-72h): 1.0 mg/L
EC50(0-72h): 6.0 mg/L (2.7-10.0)
EC20(0-72h): 2.2 mg/L (1.1-5.2)
EC10(0-72h): 1.5 mg/L (n.av.-3.0)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 56.4 mg/L
- EC10 or NOEC for freshwater algae:
- 1 mg/L
Additional information
The toxicity of test item Acid Brown 432 was tested on Pseudokirchneriella subcapitata..
A parallel test to check the colour inhibition effect was performed. The resulted % Iy shows that the inhibition is due mainly to the colour and not to toxicity of the test item:
% Iy : (72 hrs-test item): 93.2%
% Iy :(72 hrs-parallel test): 84.2%
As also stated in Regulation (EC) n. 761/2009, light absorbing test materials may give rise to a growth rate reduction because shading reduces the amount of available light. Such physical types of effects should be separated from toxic effects by modifying the test conditions and the former should be reported separately. Suggested modification by OECD Environmental Health and Safety Publications Series on Testing and Assessment No. 23, GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES, was applied.
The test can be therefore disregarded for classification.
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