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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3-bromo-5-chlorophenol
EC Number:
611-435-6
Cas Number:
56962-04-0
Molecular formula:
C6H4BrClO
IUPAC Name:
3-bromo-5-chlorophenol

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
duplicate samples were taken at the beginning and after 48 hours

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2,5 mmol/L
Test temperature:
18-22 °C with a maximum deviation of +/- 1 °C within 48 hours.
pH:
6-9; the pH-value did not vary by more than 1.5 units.
Dissolved oxygen:
> 3 mg/L
Salinity:
0,8 mmol/L
Nominal and measured concentrations:
10, 4.5, 2.1, 0.94 and 0.43 mg test item/L (nominal)
analytical measurements: 91 % of nominal concentration at test start, 94 % of nominal concentration after 48 hours
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
EC50: 1.6 mg test item/L
95 % confidenc values: not determinable
EC0: 0.94 mg test item/L
EC100: 4.5 mg test item/L
NOEC: 0.94 mg test item/L
LOEC: 2.1mg test item/L
Reported statistics and error estimates:
Calculation of EC50 values and 95 % confidence limits by Probit analysis

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
GHS relevant (EC50 <10 mg/L)