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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26 October to 05 Decemebr 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
Version / remarks:
22 July 2010
Deviations:
not specified
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Maruzen petrochemical Co Ltd. Bx 7706RXD
- Expiration date of the lot/batch: no data
- Purity test date: no data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test substance was stored in a dark freezing place.
- Stability under test conditions: Test substance readilt polymerises at ambient.
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
activated sludge, domestic
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Hardness:
No data
Test temperature:
20°C
pH:
7.5 +/- 0.5
Dissolved oxygen:
This was measured to calculate oxygen consumption rate
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
Nominal; 2.5; 6.4; 16; 39; 99 and 250 mg/L
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
240 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
< 2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of this study 3h EC 50 240 mg/L
Executive summary:

Under the conditions of this study it was found that the test substance is not toxic to microorganisms.

Description of key information

Under the conditions of this study 3h EC 50 240 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
240 mg/L
EC10 or NOEC for microorganisms:
2.5 mg/L

Additional information